Culturing bioluminescent algae requires a sample of bioluminescent algae. These organisms use photosynthesis to produce their own food and grow. They live a circadian cycle they require 12 hours of light and 12 hours of dark. The water temperature needs to be around 50°-70°F.
The algae only produce light in their night cycle and can live off a fluorescent light if natural light is not feasible or if the dark cycle is not inline with daylight.
The algae culture will last for about month. To prolong the algae purchase some growth medium. This is nutrient seawater for the algae. This algae does not require aeration of the water.